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This book is a valuable resource for students and junior technicians, as well as for qualified technologists and medical students. This Gratitude journal contains pages filled with inspirational quotes and has a great looking book cover design with a funny quote "Santa's Favorite Tech". This Journal has daily sections to write down things you are grateful for and record one daily affirmation.

This text fosters critical thinking beyond just the basic procedures, creating a thorough awareness of the clinical laboratory responsibilities that students will have to themselves, to their patients, and to the facilities where they work. Coverage includes the organization of health care facilities, the laws and regulations that govern them, and common tasks and responsibilities for the numerous professional categories that comprise the health care industry.

Safety for the laboratory employee, the patients, and the visitors are explained in detail. With an emphasis on efficiency, accuracy, and professionalism, this book serves up the essential ingredients for a holistic approach to laboratory science that augments the diagnosis and treatment of all patients.

Important Notice: Media content referenced within the product description or the product text may not be available in the ebook version. Buffers: Introduction of buffers, principle of buffering. Type and working of Buffers: acidic and alkaline buffer solutions, working of buffer solution. Preparation of buffer solution: Acetate buffer, Phosphate buffer and Tris hydroxymethyl aminomethane buffer or tris buffer.

Criteria for selection of buffers. Preparation of phosphate buffer of a desired pH. Preparation of acetate buffer of a desired pH. Clinical Laboratory equipments: Principle, working and application of spectrophotometer, colorimeter. Principle, and procedure and application of cyclomixture, centrifuge, balance, Incubator etc. Demonstration of working of colorimeter.

Demonstration of working of pH meter. Demonstration of working of incubator. Demonstration of working of cyclomixture. Demonstration of working of centrifuge, balance. Ramnik Sood, Text book of medical laboratory Technology. Godkar , Text book of medical laboratory Technology. Introduction to medical microbiology: Scope of medical microbiology. Demonstration of a bacterial cell by simple staining. Demonstration of cell wall by slide.

Demonstration of a eukaryotic cell by slide. Bacterial capsule- structure and functions. Spores- structure, functions and process of spore formation. Structure and functions cyst. Morphological and biological classification and identification of bacteria. Different forms of microorganism: L-form bacteria, spheroplast, leucoplast etc.

Bacterial inclusions bodies. Demonstration of a bacterial spore. Demonstration of flagella. Demonstration of inclusions bodies. Introduction to virology, structure of a virus and function of its components, general characteristics of viruses, symmetry of viruses.

Introduction to mycology, structure of fungi, Characteristics, morphology and classification of fungi, fungal spores. Introduction to parasitology, terminologies used in parasitology. Structure, characteristics and classification of protozoa, helminthes. Recommended Text Books 1.

Baweja, Medical Microbiology 2. Protoplasm, Structure and function of cell membrane, cytoplasm and cell organelles. Membranes and glands: Types- mucous membranes, serous and cutaneous membranes. Skin- epidermis, dermis and accessory organs.

Glands — Definition, composition and types. Chromosome- structure and function. Cell division- mitosis and meiosis. Unit-III: Thoery 2. Introduction of force, work, heat, light etc. Recommended Text Books: 1. Listening to Narratives, Listening to specific information or data, listening to Conversational contexts etc. Resume Building. Wells, Cambridge University Press. The Business Writers Handbook. Martins, Foundation Books Pvt Ltd. The students will be able to apply the knowledge of these subjects while studying the applied subjects in further semesters.

Students will also get knowledge of basic computer application. Fundamental Biochemistry-I: Introduction of chemistry, Chemical 2 1. Fundamental Biochemistry -II: 2 1. Petri plate, 2. Blood: Composition and function of blood and plasma.

Blood Volume - Normal value, determination and regulation of blood volume. Hemostasis — introduction of normal haemostasis, clotting factors, mechanism of clotting. Blood groups-A, B, O system, Rh system. Red cell indices. Lymph — lymphoid tissue formation, circulation, composition and function of lymph. Digestive System - Functions of various organs of digestive system. Salivary glands.

Gastric secretion — Composition function, regulation of gastric juice. Experiment: Practical 0. Hb estimation 0. Determination of Blood Groups 0.

Determination of body temp, Clotting Time, Bleeding Time 0. Determination of total leucocyte count 0. Determination of RBC count 0. Cardiovascular system: Heart —Properties of cardiac muscle, Cardiac cycle, Cardiac output. Physiological variations, regulation of heart rate, cardiac shock, hypotension. Pulse — Jugalar, radial pulse, Triple response. Heart sounds — Normal heart sounds, cause characteristics and signification.

Electrocardiogram ECG —significance. Blood circulation. Body temperature measurement, Physiological variation, Regulation of body temperature by physical chemical and nervous mechanisms. Role of Hypothalamus, Hypothermia and fever. Blood pressure recording 0. Auscultation for Heart Sounds 0. Measurement of body temperature. Smell —physiology, Receptors. Respiratory system: Mechanism of normal and rigorous respiration. Forces opposing and favouring expansion of the lungs.

Intra pulmonary pleural pressure, surface tension, recoil tendency of the wall. Transportation of respiratory gases. Lung volumes and capacities. Mechanisms of Regulation of respiration, Respiratory centre.

Demonstration of mechanism of respiration. Demonstration of testing of hearing. Shembulingam, Human Physiology 2. Introduction to pathology. Terminologies used in pathology, Role of hematology in diagnosis of various diseases. Laboratory organization and safety measure 2. Composition, function and of blood and plasma. Morphology of various blood cells and their identifications, abnormal morphology of red blood cells 3.

Haematopoiesis: Erythropoiesis, Leucopoiesis and Thrombopoiesis. Collection of blood by veini-puncture 0. Collection of blood by finger prick Lancet 0. Basic hematology, physiological variations, normal and absolute values. Collection of blood by vacationer. Preparation of blood smears 0. Staining of blood smears and identification of blood cells 0. Cell Injury and Cellular Adaptations: Normal cell structure and function, cell Injury- types of cell injury, etiology of cell injury.

Recommended text books: 3. Harsh Mohan, Text book of pathology. Godkar , Text book of medical laboratory Technology 5. Carbohydrate: Introduction, classification, Structure, properties and their function of monosaccharide, disaccharides and polysaccharides.

Derivatives of polysaccharides. Qualitative tests of carbohydrates by Molisch test. Qualitative tests of carbohydrates by Saliwanoff's test.

Qualitative tests of carbohydrates by Benedict test. Iodine number, sufonification, Acid value and rancidity of fat. Saturated and unsaturated fatty acid, Natural fats, Phospholipids — properties and uses. Factor affecting enzyme activity. Qualitative tests for lipids. Protein: Classification, structural organization and function of protein, peptide and sulfide bonds, Amino acid-classification, isoelectric point, optical activity and concept of pK value of amino acid.

Nucleotide, nucleoside and nitrogen bases, Role of nucleic acid, cyclic nucleotide, Synthetic nucleotides. Role of free nucleotide in biological system. Satyanarayan, Medical Biochemistry Suggested reading 1. Microscopy: - Magnification, Numerical aperture, Resolution power and component of microscope.

Use of immersion oil and care of microscope and common difficulties. Care and maintenance of microscope. Demonstration of working of microscopy. To learn the cleaning of microscope 0. To learn the use of medical microscope. Care, maintenance and validation of above instruments. To learn the use of autoclave. To learn the use of hot air oven. To learn the use of Laminar air flow. Instrumentation: Principle components, working and application of Colony counter, Mac- Intosh Field Jar, various inoculators.

Care, maintainance and validation of above instruments. Instrumentation: Petri Plate, Hot plate, distillation plant, centrifuge, Water bath, Magnetic stirrer, water bath, Deionizer etc. To learn the use of colony counter. To learn the use of magnetic stirrer. To learn the use of laminar air flow.

C Dubey, D. Windows operating system - Desktop, Start menu, Control panel, Windows accessories. Fundamentals of Computers: P. Mohan, Himalaya 4. Information Technology: Dennis P. Curtin, McGraw Hill International 5. Fundamentals of Information Technology: Saha etal, Himalaya 6.

This will help them in biochemical and microbial techniques to perform tests. Nervous system 3 2. Neuroglia, nerve fiber. Conduction of impulses continuous and saltatory. Reflex action— unconditioned properties of reflex action. Spinal cord nerve tracts. Functions of Medulla, pons.

Hypothalamic disorders. Cerebral cortex lobes and functions, Sensory cortex, Motor cortex, Cerebellum. Basal ganglion-functions.

Autonomic Nervous System 1. Menstrual cycle. Physiological changes during pregnancy. Neuromuscular junction. Transmission across the neuromuscular junction. Mechanism of muscle contraction, muscle tone, fatigue, Rigour, mortis. Skin - structure and function.

Endocrine System: Mode of action of hormones. Renal circulation peculiarities. Properties and composition of normal urine, urine output. Abnormal constituents in urine, Mechanism of urine concentration. Anaemia: Definition, classification, clinical features.

Hb estimation by CMG method. Hemoglobin determination by specific gravity method. Sickling test. Differential Leucocytes Count DLC - Principle, requirements, specimen, procedure, precautions, normal values and clinical significance. Total leucocyte count TLC. Total RBCs count. Total platelets count. Differential leucocytes count DLC. Examination of peripheral blood smears PBF. Red cell indices - significance and estimation. Deterrmination of ESR by Westergreen method. Determination of PCV. Determination of prothrombin time PT.

Godkar , Text book of medical laboratory Technology 7. Suggested Reading 2. Auto analyzer: Introduction and classification of auto analyzer, Parts of auto analyzer. Dry chemistry analyzer- principle, procedure and application. Carbohydrate metabolism: Digestion of carbohydrates, Glycolysis with clinical significance.

TCA cycle with clinical significance. Demonstration of working of Semiautoanalyzer. Demonstration of working of fully automated analyzer. Estimation of blood sugar by calorimetric method. Role of blank. Lipid metabolism: Digestion of lipid, Role of bile salt in digestion of lipid. Absorption of lipid. Hydrolysis of triacylglycerol or natural fat. Demonstration of working of calorimeter.

Demonstration of working of photometer. Demonstration of electrophoresis. Demonstration of chromatography. Demonstration of working of coulter counter. Godkar , Text book of medical laboratory Technology 3. Culture media: Common ingredients of culture media. Introduction, composition and uses of following media - peptone water, nutrient agar, nutrient broth, Mac Conkey agar, blood agar, chocolate agar, and meat extract broth etc. Special media for enterobacteriacae group, Neisseria, Corrynebacterium, Mycobacterium etc.

Cultivation of bacteria: Use of culture. Aerobic culture methods, anaerobic culture methods. Incubation Aerobic and anaerobic. Preparation of nutrient agar, Mac Conkey agar. Inoculation of NA. Preparation of agar slant. Preparation of blood agar. Identification of bacteria: A Staining for identification of bacteria: Types of staining - simple staining, negative staining, differential staining.

AFB staining- principle, preparation of stains, staining procedure. Staining procedure for - Diphtheria, spores, capsule, intracytoplasmic lipids, polysaccharides and nuclear materials. B Culture characteristics for identification of bacterial. Coagulase test, Urease test, Catalase test, oxidase test, Triple sugar iron TSI test, Nitrate reduction test, Sugar fermentation test, Litmus milk test, Methylene blue reduction test etc.

Gram staining of given culture. AFB staining of given culture. Culture characteristics of given culture. To perform IMVC pattern. Antimicrobial drug sensitivity test: Introductions, requirements, Different methods of sensitivity and interpretation of result. Pure culture: Definition, isolation of pure culture, maintenance and preservation of pure cultures. To perform antibiotic sensitivity test by KB method.

Baweja, text t book of microbiology. Tissue 2 1. Sessions Clinical Pathology-I: Physical, biochemical 3 2 0. Types of Histological preparation — Whole mount , Section, Smear etc. Responsibility of histopath technician. Fixative: Properties of fixatives, amount of fixative.

Tissue Processing: Tissue processing of histological tissues for paraffin embedding. Mould - types of moulds, techniques of casting embedding media.



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